Alexandre Marnoto de Oliveira Campos
Type of member:
PhD holder researcher
- Blue Biotechnology and Ecotoxicology - BEE
Lecturer of the discipline “Toxicogenomics and Toxicoproteomics” from the master course “Contaminação e Toxicologia Ambientais”, (ICBAS/FCUP) at University of Porto, Portugal.
Supervision and training of undergraduate and graduate students (MSc and PhD) in areas of plant physiology, environmental toxicology and biochemistry.
Organizer of the advanced course in proteomic techniques (2.5 ECTs, 28 hours). The course is a collaboration between CIIMAR and IPATIMUP institutes. 3 editions have been undertaken so far (years 2011 and 2012).
Other parallel activities include reviewer assistance in different scientific journals; assistance as jury in PhD and MSc thesis; science communication and dissemination for the general public and in the secondary schools.
CIIMAR R&D Projects
- Survey of natural substances from marine organisms with potential pharmacological and ecological applications [more info]
- Lactation and milk production in goat (Capra hircus): identifying molecular markers underlying adaptation to seasonal weight loss [more info]
- Characterization of the enzyme repertoire involved in the metabolism of toxins and pollutants in marine invertebrates [more info]
CIIMAR Publications (2007-2014)
Freitas, M, Azevedo, J, Carvalho, AP, Campos, A, Vasconcelos, V. 2014. Effects of storage, processing and proteolytic digestion on microcystin-LR concentration in edible clams. Food and Chemical Toxicology 66: 217-223. http://dx.doi.org/10.1016/j.fct.2014.01.041
Azevedo, CC, Azevedo, J, Osório, H, Vasconcelos, V, Campos, A. 2014. Early physiological and biochemical responses of rice seedlings to low concentration of microcystin-LR. Ecotoxicology 23(2): 107-121. http://dx.doi.org/10.1007/s10646-013-1156-8
Campos, A, Puerto, M, Prieto, A, Camean, A, Almeida, AM, Coelho, AV, Vasconcelos, V. 2013. Protein extraction and two-dimensional gel electrophoresis of proteins in the marine mussel Mytilus galloprovincialis: an important tool for protein expression studies, food quality and safety assessment. Journal of the Science of Food and Agriculture 93(7): 1779-1787. http://dx.doi.org/10.1002/jsfa.5977
Campos, A, Tedesco, S, Vasconcelos, V, Cristobal, S. 2012. Proteomic research in bivalves: Towards the identification of molecular markers of aquatic pollution. Journal of Proteomics . 75; 4346-435. http://dx.doi.org/10.1016/j.jprot.2012.04.027
Puerto, M, Campos, A, Prieto, A, Cameán, A, Almeida, A, Varela Coelho, A, Vasconcelos, V. 2011. Differential protein expression in two bivalve species, Mytilus galloprovincialis and Corbicula fluminea, exposed to Cylindrospermopsis raciborskii cells. Aquatic Toxicology. 101; 109-116. IF=3.333. http://dx.doi.org/10.1016/j.aquatox.2010.09.009
Prieto, A, Campos, A, Cameán, A, Vasconcelos, V. 2011. Effects on growth and oxidative stress status of rice plants (Oryza sativa) exposed to two extracts of toxin-producing cyanobacteria (Aphanizomenon ovalisporum and Microcystis aeruginosa). Ecotoxicology and Environmental Safety. 74; 1973-1980. IF=2.340. http://dx.doi.org/10.1016/j.ecoenv.2011.06.009
Campos, A, Vasconcelos, VM. 2010. Molecular mechanisms of microcystin toxicity in animals. International Journal of Molecular Sciences. 11; 268-287. IF=2.279. http://dx.doi.org/10.3390/ijms11010268
Campos, A, Carvajal-Vallejos, PK, Villalobos, E, Franco, CF, Almeida, AM, Coelho, AV, Torné, JM, Santos, M. 2010. Characterisation of Zea mays L. plastidial transglutaminase: interactions with thylakoid membrane proteins. Plant Biology. 12; 708-716. IF=2.409. http://dx.doi.org/10.1111/j.1438-8677.2009.00280.
Almeida, AM, van Harten, S, Campos, A, Coelho, AV, Cardoso, LA. 2010. The effect of weight loss on protein profiles of gastrocnemius muscle in rabbits: a study using 1D electrophoresis and peptide mass fingerprinting. Journal of Animal Physiology and Animal Nutrition. 94(2); 174-185. IF=1.106. http://dx.doi.org/10.1111/j.1439-0396.2008.00897.x
Almeida, AM, Campos, A, Francisco, R, van Harten, S, Cardoso, LA, Coelho, AV. 2010. Proteomic investigation of the effects of weight loss in the gastrocnemius muscle of wild and New Zealand white rabbits via 2D-electrophoresis and MALDI-TOF MS. Animal Genetics. 41; 260-272. IF=2.203. http://dx.doi.org/10.1111/j.1365-2052.2009.01994.x
I am working on the characterization of the bioactivity of cyanobacteria secondary metabolites at the molecular level. I am interested in theidentification of proteins that mediate/regulate cellular up-take, toxicity and bio-transformation of cyanotoxins in target organisms. This body of knowledge is important to understand the impact toxic cyanobacteria in the environment and human health. Proteomics and mass spectrometry are two important analytical tools in my research.