RISE-UP BIO is a research infrastructure initiative advancing Transnational Access for Biotechnology in Biomanufacturing. The consortium unites 24 partners from eleven EU Member States plus one associated country, representing four European research infrastructures: IBISBA, EMBRC, EuBI, and MIRRI-ERIC. Together, they provide a catalogue of over 700 services for bioprocess development, from microbial strain design to pilot-scale testing. The project addresses key European biotechnology priorities in energy, environment, chemicals, materials, health, food, and digital innovation. RISE-UP BIO supports the EU Competitiveness Compass and strengthens the European Research Area, incorporating partners from widening countries and extending services to nations like Ukraine. Eligible user entities include all engaged in biotechnology research, including commercial organizations and SMEs. SMEs benefit from an opt-out mechanism to safeguard intellectual property and EU competitive interests. Over half of the requested funding will finance user access projects, each combining multiple service modules delivered by different facilities under the governance of the four RIs. RISE-UP BIO also aims to develop an integrated R&D service catalogue, improve interoperability across life science infrastructures with metadata standards, and provide training to infrastructure staff and users to promote research quality, reproducibility, and open science. The project further explores sustainable funding models via industry and philanthropic support.