AlFunFeed aims to transform aquafeed production through innovative biotechnological processes. Its primary objective is to replace plant protein (PP) sources in aquafeeds for carnivorous fish species with single-cell protein (SCP) derived from fungal fermentation of macroalgaebased substrates. AlFunFeed is driven by a multidisciplinary approach, harnessing expertise across various fields. The initial phase lead by ODS focuses on developing SCPs from three strains of filamentous fungi (Neurospora crassa, Rhizopus oryzae, and Fusarium venenatum) and considering four variables in production (temperature, pH, oxygenation, and medium composition) resulting in 3 SCPs to replace 15% PP in diets for European seabass (Dicentrarchus labrax), as a model of carnivorous fish species. With optimal conditions defined, 3 diets will be formulated with SCP from each strain to feed juvenile seabass in a pilot study including feed trial and evaluation of immune status (CIIMAR), feed intake regulation, nutritional profiling, and digestive function (UVigo), gut microbiota (UTH), and fish welfare (UNIVPM).

The most promising SCP from pilot study will undergo a validation study under EFSA recommendations with different levels of PP replacement (15-25%) to determine optimal substitution without compromising fish robustness. Cost analysis and environmental impact of SCP and whole aquafeed production will be also evaluated. Partners of the consortium from key European aquaculture-producing countries (Greece, Spain, Italy and Portugal) representing Mediterranean and Atlantic basins, aim to generate valuable insights and data to benefit aquaculture practices globally. This collaborative effort embodies the quadruple helix model, involving academia, industry, government, and civil society to advance the aquaculture sector towards sustainability and resilience. The project emphasizes sustainability and circular economy principles by valorizing macroalgae discards as a valuable resource for SCP production. AlFunFeed contributes to development of more equitable and environmentally conscious ocean economy aligning with specific challenges outlined in the UN Decade of Ocean Science, such as sustainable ocean economy development and global food security, thus addressing critical global issues. AlFunFeed also prioritizes awareness-raising and engagement activities to promote ocean literacy and responsible aquaculture practices. Through dissemination efforts, the project aims to reach diverse audiences and foster a deeper understanding of the importance of marine resource preservation. The successful implementation of AlFunFeed could lead to the widespread adoption of sustainable aquafeed formulations on industrial scale.

Comprehensive dissemination and intellectual property management strategies are in place to ensure responsible exploitation of project outcomes, maximizing its impact and facilitating the transition towards a more sustainable aquaculture sector.